Part:BBa_K2540010
Broad host range regulatory element (strength 10)
This broad host range regulatory element consists of a constitutive promoter and a ribosome binding site which can be used for gene expression across different bacterial species. The part sequence was obtained from Johns et al. (ID 29572) and is originally found in Eubacterium rectale.
Table 1: Relative transcription and translation levels for the broad host range element (strength 10).
The part was characterized in four E. coli strains, S. oneidensis , and P. putida using mKate2 fluorescent protein. Figure 1 shows fluorescence levels after cells transformed with a plasmid containing mKate2 under the control of the broad host range regulatory element were grown in LB at 30C for 24 hours.
Figure 1: Fluorescence levels of mKate2 under the control of the broad host range element (strength 10).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3
Illegal BsaI.rc site found at 183
References
Johns, N. I. et al.(2018). Metagenomic mining of regulatory elements enables programmable species-selective gene expression. Nature Methods, 15(5), 323–329.
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